Ligation-mediated PCR improves the sensitivity of methylation analysis by restriction enzymes and detection of specific DNA strand breaks.

DNA methylation at specific sites is most frequently studied by use of methylation-sensitive restriction endonucleases and Southern blotting. We report here that the sensitivity of this method can be increased several-hundred-fold by applying a ligation-mediated polymerase chain reaction (LM-PCR) pr...

Disgrifiad llawn

Manylion Llyfryddiaeth

Cyhoeddwyd yn:	Nucleic Acids Res
Prif Awduron:	Steigerwald, S D, Pfeifer, G P, Riggs, A D
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Oxford University Press 1990
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330508/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2158077/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.6.1435
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