Ligation-mediated PCR improves the sensitivity of methylation analysis by restriction enzymes and detection of specific DNA strand breaks.

DNA methylation at specific sites is most frequently studied by use of methylation-sensitive restriction endonucleases and Southern blotting. We report here that the sensitivity of this method can be increased several-hundred-fold by applying a ligation-mediated polymerase chain reaction (LM-PCR) pr...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Steigerwald, S D, Pfeifer, G P, Riggs, A D
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1990
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330508/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2158077/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.6.1435
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