Ligation-mediated PCR improves the sensitivity of methylation analysis by restriction enzymes and detection of specific DNA strand breaks.
DNA methylation at specific sites is most frequently studied by use of methylation-sensitive restriction endonucleases and Southern blotting. We report here that the sensitivity of this method can be increased several-hundred-fold by applying a ligation-mediated polymerase chain reaction (LM-PCR) pr...
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| Publicado en: | Nucleic Acids Res |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Oxford University Press
1990
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| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330508/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2158077/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.6.1435 |
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