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Isolation of Tonoplast Vesicles from Tomato Fruit Pericarp

This protocol describes the isolation of tonoplast vesicles from tomato fruit. The vesicles isolated using this procedure are of sufficiently high purity for downstream proteomic analysis whilst remaining transport competent for functional assays. The methodology was used to study the transport of a...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Snowden, Christopher J., Thomas, Benjamin, Baxter, Charles J., Smith, J. Andrew C., Sweetlove, Lee J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5660614/
https://ncbi.nlm.nih.gov/pubmed/29085859
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.1686
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