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Isolation of Tonoplast Vesicles from Tobacco Protoplasts
Vacuoles were isolated from protoplasts of Nicotiana glutinosa by the method of Mettler and Leonard (Plant Physiol 1979 64: 1114-1120) with minor modifications so that the number of intact protoplasts contaminating the vacuole preparation was reduced to less than 1% (by number). Isopycnic centrifuga...
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| Publicado no: | Plant Physiol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1980
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440703/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661502/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.66.4.684 |
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