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Isolation of Tonoplast Vesicles from Tobacco Protoplasts

Vacuoles were isolated from protoplasts of Nicotiana glutinosa by the method of Mettler and Leonard (Plant Physiol 1979 64: 1114-1120) with minor modifications so that the number of intact protoplasts contaminating the vacuole preparation was reduced to less than 1% (by number). Isopycnic centrifuga...

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Detalles Bibliográficos
Publicado en:Plant Physiol
Main Authors: Briskin, Donald P., Leonard, Robert T.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1980
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC440703/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661502/
https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.66.4.684
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