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Isolation of Tonoplast Vesicles from Tobacco Protoplasts

Vacuoles were isolated from protoplasts of Nicotiana glutinosa by the method of Mettler and Leonard (Plant Physiol 1979 64: 1114-1120) with minor modifications so that the number of intact protoplasts contaminating the vacuole preparation was reduced to less than 1% (by number). Isopycnic centrifuga...

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Bibliografske podrobnosti
Main Authors: Briskin, Donald P., Leonard, Robert T.
Format: Artigo
Jezik:Inglês
Izdano: 1980
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC440703/
https://ncbi.nlm.nih.gov/pubmed/16661502
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