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Real-time observation of polymerase-promoter contact remodeling during transcription initiation
Critical contacts made between the RNA polymerase (RNAP) holoenzyme and promoter DNA modulate not only the strength of promoter binding, but also the frequency and timing of promoter escape during transcription. Here, we describe a single-molecule optical-trapping assay to study transcription initia...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5660091/ https://ncbi.nlm.nih.gov/pubmed/29079833 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01041-1 |
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