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Isolation of Murine Alveolar Type II Epithelial Cells
We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung...
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| Vydáno v: | Bio Protoc |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Bio-Protocol
2017
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5659635/ https://ncbi.nlm.nih.gov/pubmed/29082286 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2288 |
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