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Isolation of Murine Alveolar Type II Epithelial Cells

We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung...

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書目詳細資料
發表在:Bio Protoc
Main Authors: Sun, Fan, Xiao, Gutian, Qu, Zhaoxia
格式: Artigo
語言:Inglês
出版: Bio-Protocol 2017
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC5659635/
https://ncbi.nlm.nih.gov/pubmed/29082286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2288
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