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Isolation of Murine Alveolar Type II Epithelial Cells

We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung...

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Detalles Bibliográficos
Publicado en:Bio Protoc
Main Authors: Sun, Fan, Xiao, Gutian, Qu, Zhaoxia
Formato: Artigo
Idioma:Inglês
Publicado: Bio-Protocol 2017
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5659635/
https://ncbi.nlm.nih.gov/pubmed/29082286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2288
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