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Isolation of Murine Alveolar Type II Epithelial Cells

We have optimized a protocol for isolation of alveolar type II epithelial cells from mouse lung. Lung cell suspensions are prepared by intratracheal instillation of dispase and agarose followed by mechanical disaggregation of the lungs. Alveolar type II epithelial cells are purified from these lung...

詳細記述

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書誌詳細
出版年:Bio Protoc
主要な著者: Sun, Fan, Xiao, Gutian, Qu, Zhaoxia
フォーマット: Artigo
言語:Inglês
出版事項: Bio-Protocol 2017
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5659635/
https://ncbi.nlm.nih.gov/pubmed/29082286
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2288
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