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Evolved α‐factor prepro‐leaders for directed laccase evolution in Saccharomyces cerevisiae

Although the functional expression of fungal laccases in Saccharomyces cerevisiae has proven to be complicated, the replacement of signal peptides appears to be a suitable approach to enhance secretion in directed evolution experiments. In this study, twelve constructs were prepared by fusing native...

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Detalles Bibliográficos
Publicado en:Microb Biotechnol
Main Authors: Mateljak, Ivan, Tron, Thierry, Alcalde, Miguel
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5658585/
https://ncbi.nlm.nih.gov/pubmed/28805314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.12838
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