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Evolved α‐factor prepro‐leaders for directed laccase evolution in Saccharomyces cerevisiae

Although the functional expression of fungal laccases in Saccharomyces cerevisiae has proven to be complicated, the replacement of signal peptides appears to be a suitable approach to enhance secretion in directed evolution experiments. In this study, twelve constructs were prepared by fusing native...

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Publicat a:Microb Biotechnol
Autors principals: Mateljak, Ivan, Tron, Thierry, Alcalde, Miguel
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5658585/
https://ncbi.nlm.nih.gov/pubmed/28805314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.12838
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