Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution

Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae. Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a 22-fold increase in k(cat), the total activity was enhanced 1...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Bulter, Thomas, Alcalde, Miguel, Sieber, Volker, Meinhold, Peter, Schlachtbauer, Christian, Arnold, Frances H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC143632/
https://ncbi.nlm.nih.gov/pubmed/12571021
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.2.987-995.2003
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados