Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution

Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae. Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a 22-fold increase in k(cat), the total activity was enhanced 1...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Bulter, Thomas, Alcalde, Miguel, Sieber, Volker, Meinhold, Peter, Schlachtbauer, Christian, Arnold, Frances H.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2003
Assuntos:	Enzymology and Protein Engineering
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC143632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12571021/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.2.987-995.2003
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