Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution

Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae. Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a 22-fold increase in k(cat), the total activity was enhanced 1...

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Bibliografski detalji
Glavni autori: Bulter, Thomas, Alcalde, Miguel, Sieber, Volker, Meinhold, Peter, Schlachtbauer, Christian, Arnold, Frances H.
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2003
Teme:
Enzymology and Protein Engineering
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC143632/
https://ncbi.nlm.nih.gov/pubmed/12571021
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.2.987-995.2003
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