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Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution
Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae. Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a 22-fold increase in k(cat), the total activity was enhanced 1...
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| Glavni autori: | , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology
2003
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC143632/ https://ncbi.nlm.nih.gov/pubmed/12571021 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.2.987-995.2003 |
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