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Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution

Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae. Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a 22-fold increase in k(cat), the total activity was enhanced 1...

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Bibliografische gegevens
Hoofdauteurs: Bulter, Thomas, Alcalde, Miguel, Sieber, Volker, Meinhold, Peter, Schlachtbauer, Christian, Arnold, Frances H.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 2003
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC143632/
https://ncbi.nlm.nih.gov/pubmed/12571021
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.2.987-995.2003
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