Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution

Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae. Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a 22-fold increase in k(cat), the total activity was enhanced 1...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Bulter, Thomas, Alcalde, Miguel, Sieber, Volker, Meinhold, Peter, Schlachtbauer, Christian, Arnold, Frances H.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2003
Sujets:	Enzymology and Protein Engineering
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC143632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12571021/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.2.987-995.2003
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