Functional Expression of a Fungal Laccase in Saccharomyces cerevisiae by Directed Evolution
Laccase from Myceliophthora thermophila (MtL) was expressed in functional form in Saccharomyces cerevisiae. Directed evolution improved expression eightfold to the highest yet reported for a laccase in yeast (18 mg/liter). Together with a 22-fold increase in k(cat), the total activity was enhanced 1...
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| Veröffentlicht in: | Appl Environ Microbiol |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology (ASM)
2003
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC143632/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12571021/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.69.2.987-995.2003 |
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