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Evolved α‐factor prepro‐leaders for directed laccase evolution in Saccharomyces cerevisiae

Although the functional expression of fungal laccases in Saccharomyces cerevisiae has proven to be complicated, the replacement of signal peptides appears to be a suitable approach to enhance secretion in directed evolution experiments. In this study, twelve constructs were prepared by fusing native...

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Detalhes bibliográficos
Publicado no:Microb Biotechnol
Main Authors: Mateljak, Ivan, Tron, Thierry, Alcalde, Miguel
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5658585/
https://ncbi.nlm.nih.gov/pubmed/28805314
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.12838
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