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Refolding Active Human DNA Polymerase ν from Inclusion Bodies

Human DNA polymerase ν (Pol ν) is a conserved family A DNA polymerase of uncertain biological function. Physical and biochemical characterization aimed at understanding Pol ν function is hindered by the fact that, when over-expressed in E. coli, Pol ν is largely insoluble, and the small amount of so...

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Bibliografiske detaljer
Udgivet i:Protein Expr Purif
Main Authors: Arana, Mercedes E., Powell, Gary K., Edwards, Lori L., Kunkel, Thomas A., Petrovich, Robert M.
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5656056/
https://ncbi.nlm.nih.gov/pubmed/19853037
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.10.010
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