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Conformational heterogeneity in the Hsp70 chaperone‐substrate ensemble identified from analysis of NMR‐detected titration data

The Hsp70 chaperone system plays a critical role in cellular homeostasis by binding to client protein molecules. We have recently shown by methyl‐TROSY NMR methods that the Escherichia coli Hsp70, DnaK, can form multiple bound complexes with a small client protein, hTRF1. In an effort to characteriz...

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Détails bibliographiques
Publié dans:Protein Sci
Auteurs principaux: Sekhar, Ashok, Nagesh, Jayashree, Rosenzweig, Rina, Kay, Lewis E.
Format: Artigo
Langue:Inglês
Publié: John Wiley and Sons Inc. 2017
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5654849/
https://ncbi.nlm.nih.gov/pubmed/28833766
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3276
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