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A large‐scale expression strategy for multimeric extracellular protein complexes using Drosophila S2 cells and its application to the recombinant expression of heterodimeric ligand‐binding domains of taste receptor

Many of the extracellular proteins or extracellular domains of plasma membrane proteins exist or function as homo‐ or heteromeric multimer protein complexes. Successful recombinant production of such proteins is often achieved by co‐expression of the components using eukaryotic cells via the secreto...

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Detalles Bibliográficos
Publicado en:Protein Sci
Main Authors: Yamashita, Atsuko, Nango, Eriko, Ashikawa, Yuji
Formato: Artigo
Idioma:Inglês
Publicado: John Wiley and Sons Inc. 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5654844/
https://ncbi.nlm.nih.gov/pubmed/28833672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3271
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