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GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs

Applications of the GFP-fusion technique have greatly facilitated evaluations of the amounts and qualities of sample proteins used for structural analyses. In this study, we applied the GFP-based sample evaluation to secreted protein expression by insect cells. We verified that a GFP variant, GFPuv,...

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Bibliografische gegevens
Hoofdauteurs: Ashikawa, Yuji, Ihara, Makoto, Matsuura, Noriko, Fukunaga, Yuko, Kusakabe, Yuko, Yamashita, Atsuko
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Wiley Subscription Services, Inc., A Wiley Company 2011
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3218366/
https://ncbi.nlm.nih.gov/pubmed/21805523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.707
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