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Rapid kinetic BRET measurements to monitor G protein activation by GPCR and non-GPCR proteins
Heterotrimeric G proteins are central hubs of signal transduction whose activity is controlled by G-protein coupled receptors (GPCRs) as well as by a complex network of regulatory proteins. Recently, Bioluminescence Resonance Energy Transfer (BRET)-based assays have been used to monitor real-time ac...
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| Publicado no: | Methods Cell Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5654623/ https://ncbi.nlm.nih.gov/pubmed/28964333 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/bs.mcb.2017.08.001 |
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