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A Genetically Encoded Cyclobutene Probe for Labelling of Live Cells
We have identified an aminoacyl-tRNA synthetase/tRNA pair for the efficient and site-specific incorporation of a cyclobutene-containing amino acid into proteins in response to amber nonsense codon. Fast and fluorescent labeling of purified proteins and intact proteins in live cells was demonstrated...
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| Publicado no: | Chem Commun (Camb) |
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| Main Authors: | , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5648060/ https://ncbi.nlm.nih.gov/pubmed/28902227 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7cc05580c |
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