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Simple Meets Single: The Application of CRISPR/Cas9 in Haploid Embryonic Stem Cells

The CRISPR/Cas9 system provides a powerful method for the genetic manipulation of the mammalian genome, allowing knockout of individual genes as well as the generation of genome-wide knockout cell libraries for genetic screening. However, the diploid status of most mammalian cells restricts the appl...

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Bibliografiske detaljer
Udgivet i:Stem Cells Int
Main Authors: Yin, Zixi, Chen, Lingyi
Format: Artigo
Sprog:Inglês
Udgivet: Hindawi 2017
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5646320/
https://ncbi.nlm.nih.gov/pubmed/29109740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2017/2601746
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