Characterization of Fluorescein Arsenical Hairpin (FlAsH) as a Probe for Single-Molecule Fluorescence Spectroscopy

In recent years, new labelling strategies have been developed that involve the genetic insertion of small amino-acid sequences for specific attachment of small organic fluorophores. Here, we focus on the tetracysteine FCM motif (FLNCCPGCCMEP), which binds to fluorescein arsenical hairpin (FlAsH), an...

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Veröffentlicht in:Sci Rep
Hauptverfasser: Fernandes, Dennis D., Bamrah, Jasbir, Kailasam, Senthilkumar, Gomes, Gregory-Neal W., Li, Yuchong, Wieden, Hans-Joachim, Gradinaru, Claudiu C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Publishing Group UK 2017
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5638890/
https://ncbi.nlm.nih.gov/pubmed/29026195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-13427-8
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