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Generation, Diversity Determination, and Application to Antibody Selection of a Human Naïve Fab Library

We constructed a large naïve human Fab library (3 × 10(10) colonies) from the lymphocytes of 809 human donors, assessed available diversities of the heavy-chain variable (VH) and κ light-chain variable (VK) domain repertoires, and validated the library by selecting Fabs against 10 therapeutically re...

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Publicat a:Mol Cells
Autors principals: Kim, Sangkyu, Park, Insoo, Park, Seung Gu, Cho, Seulki, Kim, Jin Hong, Ipper, Nagesh S., Choi, Sun Shim, Lee, Eung Suk, Hong, Hyo Jeong
Format: Artigo
Idioma:Inglês
Publicat: Korean Society for Molecular and Cellular Biology 2017
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5638773/
https://ncbi.nlm.nih.gov/pubmed/28927259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.14348/molcells.2017.0106
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