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Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Demand for monoclonal antibodies (mAbs) in basic research and medicine is increasing yearly. Hybridoma technology has been the dominant method for mAb development since its first report in 1975. As an alternative technology, phage display methods for mAb development are increasingly attractive since...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Huang, Ganggang, Zhong, Zhenwei, Miersch, Shane, Sidhu, Sachdev S., Hou, Shin-chen, Wu, Donghui
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2018
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101057/
https://ncbi.nlm.nih.gov/pubmed/29782009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57357
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