Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage.

We have developed efficient methodologies for construction and expression of comprehensive phage display libraries of murine Fab antibody fragments in E. coli cells. Our methods optimize several critical steps of the polymerase chain reaction (PCR) amplification of transcripts of the re-arranged imm...

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Hauptverfasser: Orum, H, Andersen, P S, Oster, A, Johansen, L K, Riise, E, Bjørnvad, M, Svendsen, I, Engberg, J
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1993
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC311180/
https://ncbi.nlm.nih.gov/pubmed/8233782
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