Efficient method for constructing comprehensive murine Fab antibody libraries displayed on phage.

We have developed efficient methodologies for construction and expression of comprehensive phage display libraries of murine Fab antibody fragments in E. coli cells. Our methods optimize several critical steps of the polymerase chain reaction (PCR) amplification of transcripts of the re-arranged imm...

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Dettagli Bibliografici

Pubblicato in:	Nucleic Acids Res
Autori principali:	Orum, H, Andersen, P S, Oster, A, Johansen, L K, Riise, E, Bjørnvad, M, Svendsen, I, Engberg, J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1993
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC311180/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8233782/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/21.19.4491
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