Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization

We present a CRISPR/Cas9 genome-editing strategy to systematically tag endogenous proteins with fluorescent tags in human induced pluripotent stem cells (hiPSC). To date, we have generated multiple hiPSC lines with monoallelic green fluorescent protein tags labeling 10 proteins representing major ce...

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Veröffentlicht in:Mol Biol Cell
Hauptverfasser: Roberts, Brock, Haupt, Amanda, Tucker, Andrew, Grancharova, Tanya, Arakaki, Joy, Fuqua, Margaret A., Nelson, Angelique, Hookway, Caroline, Ludmann, Susan A., Mueller, Irina A., Yang, Ruian, Horwitz, Rick, Rafelski, Susanne M., Gunawardane, Ruwanthi N.
Format: Artigo
Sprache:Inglês
Veröffentlicht: The American Society for Cell Biology 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5638588/
https://ncbi.nlm.nih.gov/pubmed/28814507
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E17-03-0209
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