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Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures
The generation of high-quality protein crystals and the loss of phase information during an X-ray crystallography diffraction experiment represent the major bottlenecks in the determination of novel protein structures. A generic method for introducing Hg atoms into any crystal independent of the pre...
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| Publicat a: | Acta Crystallogr D Struct Biol |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
International Union of Crystallography
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5633906/ https://ncbi.nlm.nih.gov/pubmed/28994409 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2059798317013171 |
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