Directed differentiation and long-term maintenance of epicardial cells from human pluripotent stem cells under fully defined conditions

The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to self-renewing epicardial cells in a completely defined, xeno-free system by temporal modulation of regulators of canonical Wnt signaling. Appropriate differentiation stage-specific application of Gsk3 inhibitor,...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Bao, Xiaoping, Lian, Xiaojun, Qian, Tongcheng, Bhute, Vijesh J., Han, Tianxiao, Palecek, Sean P.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5630264/
https://ncbi.nlm.nih.gov/pubmed/28817124
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.080
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