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Directed differentiation and long-term maintenance of epicardial cells from human pluripotent stem cells under fully defined conditions
The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to self-renewing epicardial cells in a completely defined, xeno-free system by temporal modulation of regulators of canonical Wnt signaling. Appropriate differentiation stage-specific application of Gsk3 inhibitor,...
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| Publicado no: | Nat Protoc |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5630264/ https://ncbi.nlm.nih.gov/pubmed/28817124 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.080 |
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