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The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging

Multicellular tubes, fundamental units of all internal organs, are composed of polarized epithelial or endothelial cells, with apical membranes lining the lumen and basolateral membranes contacting each other and/or the extracellular matrix. How this distinctive membrane asymmetry is established and...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Zhang, Nan, Khan, Liakot A, Membreno, Edward, Jafari, Gholamali, Yan, Siyang, Zhang, Hongjie, Gobel, Verena
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5628585/
https://ncbi.nlm.nih.gov/pubmed/28994799
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56100
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