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RNAi-based biosynthetic pathway screens to identify in vivo functions of non-nucleic-acid-based metabolites such as lipids

The field of metabolomics continues to catalog new compounds, but their functional analysis remains technically challenging, and roles beyond metabolism are largely unknown. Unbiased genetic/RNAi screens are powerful tools to identify the in vivo functions of protein-encoding genes, but not of non-p...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Zhang, Hongjie, Abraham, Nessy, Khan, Liakot A., Gobel, Verena
Formato: Artigo
Idioma:Inglês
Publicado em: 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5597045/
https://ncbi.nlm.nih.gov/pubmed/25837419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2015.031
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