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Using competition assays to quantitatively model cooperative binding by transcription factors and other ligands

BACKGROUND: The affinities of DNA binding proteins for target sites can be used to model the regulation of gene expression. These proteins can bind to DNA cooperatively, strongly impacting their affinity and specificity. However, current methods for measuring cooperativity do not provide the means t...

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Detalhes bibliográficos
Publicado no:Biochim Biophys Acta
Main Authors: Peacock, Jacob, Jaynes, James B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5623634/
https://ncbi.nlm.nih.gov/pubmed/28774855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbagen.2017.07.024
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