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Loquacious-PD facilitates Drosophila Dicer-2 cleavage through interactions with the helicase domain and dsRNA

Loquacious-PD (Loqs-PD) is required for biogenesis of many endogenous siRNAs in Drosophila. In vitro, Loqs-PD enhances the rate of dsRNA cleavage by Dicer-2 and also enables processing of substrates normally refractory to cleavage. Using purified components, and Loqs-PD truncations, we provide a mec...

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Vydáno v:Proc Natl Acad Sci U S A
Hlavní autoři: Trettin, Kyle D., Sinha, Niladri K., Eckert, Debra M., Apple, Sarah E., Bass, Brenda L.
Médium: Artigo
Jazyk:Inglês
Vydáno: National Academy of Sciences 2017
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5617286/
https://ncbi.nlm.nih.gov/pubmed/28874570
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1707063114
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