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Dicer's helicase domain discriminates dsRNA termini to promote an altered reaction mode
The role of Dicer's helicase domain is enigmatic, but in vivo it is required for processing certain endogenous siRNA, but not miRNA. Using C. elegans extracts, or purified Drosophila Dicer-2, we compared activities of wildtype enzymes and those containing mutations in the helicase domain. We fo...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3061311/ https://ncbi.nlm.nih.gov/pubmed/21362554 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.molcel.2011.02.005 |
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