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Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
We present a simple method for efficient DNA ligation utilizing the heat generation of ferromagnetic particles subjected to an ac magnetic field. We carry out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on the surface of ferromagnetic particles. When a radio freq...
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| Publicado en: | Biochem Biophys Rep |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Elsevier
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5614472/ https://ncbi.nlm.nih.gov/pubmed/28955977 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2016.10.006 |
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