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Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field
We present a simple method for efficient DNA ligation utilizing the heat generation of ferromagnetic particles subjected to an ac magnetic field. We carry out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on the surface of ferromagnetic particles. When a radio freq...
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| Yayımlandı: | Biochem Biophys Rep |
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| Asıl Yazarlar: | , , , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
Elsevier
2016
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5614472/ https://ncbi.nlm.nih.gov/pubmed/28955977 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2016.10.006 |
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