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Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field

We present a simple method for efficient DNA ligation utilizing the heat generation of ferromagnetic particles subjected to an ac magnetic field. We carry out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on the surface of ferromagnetic particles. When a radio freq...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Rep
Main Authors: Suzuki, Masashi, Hayashi, Hiroaki, Mizuki, Toru, Maekawa, Toru, Morimoto, Hisao
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614472/
https://ncbi.nlm.nih.gov/pubmed/28955977
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2016.10.006
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