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Simultaneous Measurement of HDAC1 and HDAC6 Activity in HeLa Cells Using UHPLC-MS

The search for new histone deacetylase (HDAC) inhibitors is of increasing interest in drug discovery. Isoform selectivity has been in the spotlight since the approval of romidepsin, a class I HDAC inhibitor for cancer therapy, and the clinical investigation of HDAC6-specific inhibitors for multiple...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Simões-Pires, Claudia A., Zwick, Vincent, Cretton, Sylvian, Cuendet, Muriel
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614247/
https://ncbi.nlm.nih.gov/pubmed/28829415
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55878
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