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Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator

Epithelial ion transport is vital to systemic ion homeostasis as well as maintenance of essential cellular electrochemical gradients. Intracellular pH (pH(i)) is influenced by many ion transporters and thus monitoring pH(i )is a useful tool for assessing transporter activity. Modern Genetically Enco...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Rossano, Adam J., Romero, Michael F.
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2017
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614243/
https://ncbi.nlm.nih.gov/pubmed/28829430
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55698
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