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Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator
Epithelial ion transport is vital to systemic ion homeostasis as well as maintenance of essential cellular electrochemical gradients. Intracellular pH (pH(i)) is influenced by many ion transporters and thus monitoring pH(i )is a useful tool for assessing transporter activity. Modern Genetically Enco...
Enregistré dans:
| Publié dans: | J Vis Exp |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5614243/ https://ncbi.nlm.nih.gov/pubmed/28829430 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55698 |
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