Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Prenyltransferases (PT) are a group of enzymes that catalyze chain elongation of allylic diphosphate using isopentenyl diphosphate (IPP) via multiple condensation reactions. DHDDS (dehydrodolichyl diphosphate synthase) is a eukaryotic long-chain cis-PT (forming cis double bonds from the condensation...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Edri, Ilan, Goldenberg, Michal, Lisnyansky, Michal, Strulovich, Roi, Newman, Hadas, Loewenstein, Anat, Khananshvili, Daniel, Giladi, Moshe, Haitin, Yoni
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5614002/
https://ncbi.nlm.nih.gov/pubmed/28809830
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56430
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