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Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
Prenyltransferases (PT) are a group of enzymes that catalyze chain elongation of allylic diphosphate using isopentenyl diphosphate (IPP) via multiple condensation reactions. DHDDS (dehydrodolichyl diphosphate synthase) is a eukaryotic long-chain cis-PT (forming cis double bonds from the condensation...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5614002/ https://ncbi.nlm.nih.gov/pubmed/28809830 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56430 |
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