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A high yield optimized method for the production of acylated ACPs enabling the analysis of enzymes involved in P. falciparum fatty acid biosynthesis

The natural substrates of the enzymes involved in type-II fatty acid biosynthesis (FAS-II) are acylated acyl carrier proteins (acyl-ACPs). The state of the art method to produce acyl-ACPs involves the transfer of a phosphopantetheine moiety from CoA to apo-ACP by E. coli holo-ACP synthase (EcACPS),...

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Detalhes bibliográficos
Publicado no:Biochem Biophys Rep
Main Authors: Lauciello, Leonardo, Lack, Gabriela, Scapozza, Leonardo, Perozzo, Remo
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5613970/
https://ncbi.nlm.nih.gov/pubmed/28955970
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2016.09.017
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