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Application of a novel HiBiT peptide tag for monitoring ATF4 protein expression in Neuro2a cells
A split NanoLuc assay system consisting of two fragments, large N-terminal and small C-terminal regions (NanoBiT), was developed to investigate protein-protein interactions within living cells. Interestingly, the replacement of five amino acids among 11 C-terminal amino acids dramatically increased...
Enregistré dans:
| Publié dans: | Biochem Biophys Rep |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5613219/ https://ncbi.nlm.nih.gov/pubmed/28955790 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2017.08.002 |
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