Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Moloney murine leukemia (MLV) virus-based retroviral vectors integrate predominantly in acetylated enhancers and promoters. For this reason, mLV integration sites can be used as functional markers of active regulatory elements. Here, we present a retroviral scanning tool, which allows the genome-wid...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Romano, Oriana, Cifola, Ingrid, Poletti, Valentina, Severgnini, Marco, Peano, Clelia, De Bellis, Gianluca, Mavilio, Fulvio, Miccio, Annarita
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2017
Fag:
Genetics
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608156/
https://ncbi.nlm.nih.gov/pubmed/28605390
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55919
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