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Locus-specific integration of extrachromosomal transgenes in C. elegans with the CRISPR/Cas9 system

We established a method to generate integration from extrachromosomal arrays with the CRISPR/Cas9 system. Multi-copy transgenes were integrated into the defined loci of chromosomes by this method, while a multi-copy transgene is integrated into random loci by previous methods, such as UV- and gamma-...

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Publicat a:Biochem Biophys Rep
Autors principals: Yoshina, Sawako, Suehiro, Yuji, Kage-Nakadai, Eriko, Mitani, Shohei
Format: Artigo
Idioma:Inglês
Publicat: Elsevier 2015
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5600330/
https://ncbi.nlm.nih.gov/pubmed/28955808
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrep.2015.11.017
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