Identification of Vaccinia Virus Replisome and Transcriptome Proteins by Isolation of Proteins on Nascent DNA Coupled with Mass Spectrometry

Poxviruses replicate within the cytoplasm and encode proteins for DNA and mRNA synthesis. To investigate poxvirus replication and transcription from a new perspective, we incorporated 5-ethynyl-2′-deoxyuridine (EdU) into nascent DNA in cells infected with vaccinia virus (VACV). The EdU-labeled DNA w...

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Bibliographic Details
Published in:J Virol
Main Authors: Senkevich, Tatiana G., Katsafanas, George C., Weisberg, Andrea, Olano, Lisa R., Moss, Bernard
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2017
Subjects:
Genome Replication and Regulation of Viral Gene Expression
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5599757/
https://ncbi.nlm.nih.gov/pubmed/28747503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.01015-17
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