Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms

High-level, recombinant production of membrane-integrated proteins in Escherichia coli is extremely relevant for many purposes, but has also been proven challenging. Here we study a combination of transcriptional fine-tuning in E. coli LEMO21(DE3) with different codon usage algorithms for heterologo...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Claassens, Nico J., Siliakus, Melvin F., Spaans, Sebastiaan K., Creutzburg, Sjoerd C. A., Nijsse, Bart, Schaap, Peter J., Quax, Tessa E. F., van der Oost, John
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2017
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5597330/
https://ncbi.nlm.nih.gov/pubmed/28902855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0184355
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