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Heterologous Protein Expression Is Enhanced by Harmonizing the Codon Usage Frequencies of the Target Gene with those of the Expression Host

Synonymous codon replacement can change protein structure and function, indicating that protein structure depends on DNA sequence. During heterologous protein expression, low expression or formation of insoluble aggregates may be attributable to differences in synonymous codon usage between expressi...

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Detalhes bibliográficos
Main Authors: Angov, Evelina, Hillier, Collette J., Kincaid, Randall L., Lyon, Jeffrey A.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2364656/
https://ncbi.nlm.nih.gov/pubmed/18478103
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0002189
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