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Chromosome conformation and gene expression patterns differ profoundly in human fibroblasts grown in spheroids versus monolayers
Human cells derived for in vitro cultures are conventionally grown as adherent monolayers (2D) which do not resemble natural 3 dimensional (3D) tissue architecture. We examined genome structure with chromosome conformation capture (Hi-C) and gene expression with RNA-seq in fibroblasts derived from h...
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| Publicado no: | Nucleus |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5597289/ https://ncbi.nlm.nih.gov/pubmed/28129029 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/19491034.2017.1280209 |
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